There is however, no guarantee that the female will be receptive. Females might reject males for a number of reasons; he just might not look right (being a different subspecies perhaps); he might be too small (she may be seeking a large spermatophore produced by a large male); he might not smell right (the sex pheromones and volatile cuticular hydrocarbons); or she may have already mated (1). Bad luck! She indicates her refusal by bending her abdomen in a certain way and flapping her wings.
Source: Tigers mating